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KMID : 1134820170460070886
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 7 p.886 ~ p.890
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Neuroprotective Effect of Rice with Phellinus linteus Mycelium in HT22 Cells
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Kim Ji-Hyun
Chun Soon-Sil
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Abstract
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In this study, the protective effect of rice with Phellinus linteus mycelium (PLMR) against hydrogen peroxide-induced oxidative stress was assessed in a mouse hippocampal neuronal HT22 cell line through (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) salt (MTS) assay and western blot. MTS assay using HT22 cells showed that PLMR extract did not affect viability at a concentration range from 1 mg/mL to 5 mg/mL. However, at concentrations over 10 mg/mL, PLMR extract resulted in increased cell death. Cell viability of HT22 was significantly reduced by H2O2 treatment, and reduction of cell viability was efficiently restored by treatment with PLMR extract in a dose-dependent manner from 0.1 to 1 mg/mL. Cells treated with H2O2 showed increased expression of Bax, a pro-apoptotic protein, which was down-regulated by treatment with PLMR extract. On the other hand, cells treated with H2O2 resulted in reduced expression of Bcl-2, an anti-apoptotic protein, which was restored by treatment with PLMR extract. In addition, treatment with PLMR extract reduced expression of cleaved caspase 3 and PARP, which were up-regulated by H2O2 treatment. The results may suggest that treatment with PLMR extract would suppress H2O2-induced apoptosis of HT22 cells.
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KEYWORD
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Phellinus linteus mycelium rice, HT22, oxidative stress, MTS assay, western blot
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